Method validated for selected mono- and di-oxygenated metabolites of arachidonic acid in the cytochrome P450 and lipoxygenase pathways:
- Hydroxyeicosatetraenoic acid (HETE) metabolites (12-HETE, 15-HETE, 20-HETE (shown above))
- Epoxyeicosatrienoic acid (EET) metabolites (8,9 – EET, 11,12 – EET, 14,15 – EET)
- Dihydroxyeicosatrienoic acid (DiHETrE) metabolites (5,6 – DiHETrE, 8,9 – DiHETrE, 11,12 – DiHETrE, 14,15 – DiHETrE)
- Linoleic acid metabolites (9,10 – DiHOME, 9,10 – EpOME)
Typical sample volume: 1.0 mL human cerebrospinal fluid (CSF); 0.5 mL human plasma
Typical Lower Limit of Quantitation: 0.2 ng/mL in human CSF and rat brain; 0.4 ng/mL in human plasma