Method validated for selected metabolites of arachidonic acid in the cyclooxygenase pathways:
- Prostaglandin A2 (PGA2)
- Prostaglandin D2 (PGD2): 15-deoxy PGD2
- Prostaglandin E2 (PGE2, shown above)
- Prostaglandin F1alpha (PGF1alpha): 6-keto PGF1alpha
- Prostaglandin F2alpha (PGF2alpha): 11-beta PGF2alpha
- Prostaglandin J2 (PGJ2): 15-deoxy PGJ2, Delta-12 PGJ2
- 11-dehydro thromboxane B2
Typical sample volume: Rat brain cortical tissue; 0.5 mL human plasma
Typical Lower Limit of Quantitation: 0.1-0.2 ng/mL in rat brain cortical tissue; 0.4 ng/mL in human plasma